Manual for topoisomerase ii drug screening kit




















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Coord Chem Rev — Google Scholar. Chin Tradi Pat Med — CAS Google Scholar. Book Google Scholar. Madden KR, Champoux JJ Overexpression of human topoisomerase I in baby hamster kidney cells: hypersensitivity of clonal isolates to camptothecin.

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Exp Opin Drug Deliv — Rating Required Select Rating 1 star worst 2 stars 3 stars average 4 stars 5 stars best. Email Required. Review Subject Required. Comments Required. Current Stock:. Quantity: Decrease Quantity: Increase Quantity:. Forward to a Friend. Format Well Plate. Intra-assay Precision Precision within an assay : 3 samples with low, middle and high level Topoisomerase II TOP2 were tested 20 times on one plate, respectively. Inter-assay Precision Precision between assays : 3 samples with low, middle and high level Topoisomerase II TOP2 were tested on 3 different plates, 8 replicates in each plate.

The test principle applied in this kit is Sandwich enzyme immunoassay. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase II TOP2. Prepare all reagents, samples and standards; 2. Aspirate and wash 3 times; 5. Aspirate and wash 5 times; 7. The kit will allow detection of novel compounds that either stabilize the nicked intermediate or otherwise inhibit catalytic activity of topoisomerase I.

Description of Product. The reactions were incubated for 30 min at 37 C and stopped with SDS.



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